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Human monocyte CD14 is upregulated by lipopolysaccharide
R Landmann1, H P Knopf, S Link
1Department of Research and Internal Medicine, University Hospital, Basel, Switzerland.
Infection and Immunity
|May 1, 1996
Summary
Lipopolysaccharide (LPS) upregulates CD14 receptor expression in human monocytes and macrophages. This transcriptional upregulation of CD14 mRNA and membrane CD14 (mCD14) occurs after prolonged LPS exposure and is mediated by bacterial cell wall components.
Area of Science:
- Immunology
- Cell Biology
Background:
- Membrane CD14 (cluster of differentiation 14) is crucial for monocyte activation by lipopolysaccharide (LPS).
- The in vitro regulation of CD14 by LPS remains largely uncharacterized.
Purpose of the Study:
- To investigate the effects of LPS on CD14 mRNA and protein levels (membrane-bound CD14 [mCD14] and soluble CD14 [sCD14]) in human monocytes and macrophages.
Main Methods:
- Human monocytes and macrophages were stimulated with LPS in vitro.
- CD14 mRNA and protein expression, as well as LPS binding, were analyzed over time.
- Effects of various bacterial components and blocking antibodies were assessed.
Main Results:
- LPS induced a late, transcriptional upregulation of CD14 mRNA and mCD14 after 2-day incubation.
- Soluble CD14 (sCD14) release was transiently enhanced.
- Other bacterial cell wall components also increased mCD14, and the LPS effect was blocked by polymyxin B.
Conclusions:
- CD14 is transcriptionally upregulated by LPS and other bacterial cell wall constituents.
- This upregulation is a late response and distinct from early inflammatory cytokine production.