Hepatic uptake and biliary excretion of a bile salt analog : glycodihydrofusidate

Glyco-24, 25-dihydrofusidate is efficiently excreted in the bile by the liver. This compound partially inhibits hepatobiliary transport of bile salts and bilirubin. In order to determine if bile salts and glycodihydrofusidate share common pathways at the hepatocyte level, we studied the effect of sodium dehydrocholate on the blood clearance of glycodihydrofusidate. Two groups of rats were perfused, controls with 0.15M NaCl, and test animals with 10 micronmol + min-(1) + kg(1) of dehydrocholate; both groups received 1 micronCi of 14C-glycodihydrofusidate intravenously. Carotid blood was removed every minute and the disappearance of radioactivity from the blood was monitored. The results are consisgent with a theoretical model of two compartments. The experimental curves were fit to the sum of exponentials.