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Recombinant insulin-like growth factor-1 modulates aggregation in human platelets via extracellular calcium
A S Motani1, E E Anggård, G A Ferns
1William Harvey Research Institute, London, U.K.
Life Sciences
|January 1, 1996
Abstract:
Insulin like growth factor-1 (IGF-1) potentiated aggregation of human platelets induced by thrombin-, collagen- and ADP in a dose-dependent manner over the range 30-300 nM. IGF-1 (100 nM) reduced EC50 values for thrombin, collagen and ADP-induced aggregation by 19.6%, 53.6% and 22.8% respectively. Potentiation by IGF-1 was dependent on the presence of extracellular Ca2+ and was inhibited by verapamil or nifedipine. Further, IGF-1 enhanced the elevation in free intraplatelet Ca2+ induced by the platelet agonists collagen and thrombin.