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Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

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Published on: December 14, 2015

Direct and long-range action of a DPP morphogen gradient

D Nellen¹, R Burke, G Struhl

¹Zoologisches Institut, Universität Zürich, Switerland.

|May 3, 1996

Summary

Decapentaplegic (DPP) protein acts directly over long distances to organize Drosophila wing development. Different concentrations of DPP activate specific genes, suggesting it functions as a gradient morphogen.

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Last Updated: Jun 27, 2026

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Imaging Dpp Release from a Drosophila Wing Disc

Imaging Dpp Release from a Drosophila Wing Disc

Published on: October 30, 2019

Area of Science:

Developmental biology
Genetics
Molecular biology

Background:

The decapentaplegic (DPP) gene is crucial for Drosophila wing development.
DPP protein is secreted from a specific cell stripe and influences distant cells.

Purpose of the Study:

To investigate the mechanism of DPP action during Drosophila wing development.
To determine if DPP acts directly or indirectly through other signaling molecules.
To understand how DPP concentration gradients affect gene expression.

Main Methods:

Utilizing genetically modified cell clones in Drosophila wings.
Manipulating DPP receptor activity (constitutive activation or abolition).
Analyzing the transcriptional activation of downstream genes (optomotor-blind and spalt).

Main Results:

DPP acts directly on responding cells at long range.
DPP does not act indirectly via short-range signaling molecules.
Optomotor-blind and spalt genes are transcriptionally activated at different distances from DPP-secreting cells.
Evidence suggests differential gene response to varying DPP concentrations.

Conclusions:

DPP functions as a long-range morphogen during Drosophila wing development.
Gene activation by DPP depends on concentration thresholds.
This provides a model for understanding morphogen gradients in development.