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Updated: Feb 9, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
[Studies on the contents of nitrates and nitrites in selected fresh and heat processed vegetables]
A Markowska1, A Kotkowska, W Furmanek
1Centralnego Ośrodka Badawczo-Rozwojowego Przemysłu Gastronomicznego i Artykułów Spozywczych, Centralne Laboratorium Jakości Lodzi.
Abstract:
The levels of nitrates and nitrites were determined in fresh vegetables and the same products subjected to culinary processing such as boiling. Nitrates were reduced on a cadmium column to nitrites, where upon they were determined colorimetrically using sulfanilic acid and N-1-naphthyl-ethylenediamine. Thermal processing of these vegetables reduced the level of nitrates by about 50% and the nitrites loss reached even 100%.
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