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Related Experiment Videos

High-throughput screen for detecting antimycobacterial agents

G A Chung1, Z Aktar, S Jackson

  • 1Glaxo Research and Development Ltd., Stevenage, Hertfordshire, United Kingdom.

Antimicrobial Agents and Chemotherapy
|October 1, 1995
PubMed
Summary
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A new assay system screens for drugs that inhibit mycobacterial growth. It uses Mycobacterium aurum to predict activity against Mycobacterium tuberculosis, enabling rapid testing of thousands of samples daily.

Area of Science:

  • Microbiology
  • Drug Discovery
  • Assay Development

Background:

  • Screening for novel anti-mycobacterial agents is crucial for combating tuberculosis.
  • Mycobacterium tuberculosis presents significant challenges for drug screening due to its slow growth and infectious nature.

Purpose of the Study:

  • To develop a simple, robust, and high-throughput assay for identifying inhibitors of mycobacterial growth.
  • To establish a predictive model for anti-mycobacterial activity using a surrogate organism.

Main Methods:

  • Utilized Mycobacterium aurum, a rapidly growing saprophyte, as a model organism for screening.
  • Assessed cellular viability by measuring radiolabeled uracil uptake in M. aurum.
  • Implemented a microtiter plate format for large-scale sample testing.

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Main Results:

  • Developed a reliable assay system for screening mycobacterial growth inhibitors.
  • Demonstrated that M. aurum growth inhibition is predictive of activity against M. tuberculosis.
  • The assay system allows for the testing of thousands of samples per day.

Conclusions:

  • The developed assay provides a scalable and efficient platform for anti-mycobacterial drug discovery.
  • This method overcomes the limitations associated with using M. tuberculosis directly in screening.
  • The assay system holds significant potential for accelerating the identification of new tuberculosis treatments.