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Introducing StuI sites improves vectors for the expression of fusion proteins with factor Xa cleavage sites
1Institute of Plant Sciences, Swiss Federal Institute of Technology, Zürich, Switzerland.
Gene
|April 17, 1996
Abstract:
The Arg-encoding triplet (AGG) in the recognition sequence Ile-Glu-Gly-Arg for factor Xa can be used to generate a StuI restriction site (AGGCCT) which greatly facilitates the construction of DNA fragments encoding fusion proteins. Following proteolytic cleavage with factor Xa, a protein with the desired N terminus can be obtained.