[Herpes simplex virus and ovarian malignancies]

Immunologic methods were used to examine 35 sera and the same number of malignant tissue samples taken from female patients with verified ovarian malignomas in order to establish presence of herpes simplex virus. In 23 preoperative sera presence of herpes simplex virus antibodies was established. In 23 postoperatively taken sera there was an evident decrease of antibody titres. In both samples antibodies were IgG class. In 25 samples of malignant tissues presence of herpes simplex virus antigen was established by method of indirect imunnofluorescency. In 9 samples antigen was of the type 1. In 6 cases it was herpes simplex virus of type 2. In order to isolate the complete infective virion, acellular filtrates taken from malignant tissues were used, inoculated with Vero cell cultures, as well as experimental intracerebrally inoculated mice. Cytopathogenic effect on cell cultures was typical but poor and it disappeared in the following passages. In inoculated mice symptoms were also typical, but incomplete. In the examined brains dyed by indirect imunnofluorescency method only focal fluorescence being different from diffuse which characterizes the complete infective virus, was established.