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TGF-beta signals and a pattern in Xenopus laevis endodermal development

G L Henry1, I H Brivanlou, D S Kessler

  • 1Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Development (Cambridge, England)
|March 1, 1996
PubMed
Summary
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Early Xenopus endoderm development involves signaling pathways. TGF-beta signaling influences germ layer segregation and the expression of pancreas and intestinal markers.

Area of Science:

  • Developmental Biology
  • Xenopus laevis Embryogenesis
  • Molecular Signaling Pathways

Background:

  • Early endoderm development in Xenopus laevis involves the expression of specific gene products.
  • Pancreas development is marked by Xlhbox-8, while intestinal fatty acid binding protein (IFABP) marks the small intestinal epithelium.

Purpose of the Study:

  • To investigate the signaling mechanisms regulating gene expression in the early Xenopus endoderm.
  • To determine the role of transforming growth factor-beta (TGF-beta) and fibroblast growth factor (FGF) signaling in endodermal patterning.
  • To explore the relationship between endodermal patterning and mesodermal development signals.

Main Methods:

  • Analysis of gene expression patterns for Xlhbox-8 and IFABP in early Xenopus endoderm.

Related Experiment Videos

  • Utilized endodermal explants devoid of mesoderm.
  • Investigated the role of cortical rotation and TGF-beta signaling inhibition.

    • Main Results:

    • Pancreas marker (Xlhbox-8) expression depends on TGF-beta and FGF signaling.
    • Small intestinal marker (IFABP) expression is independent of these signals.
    • Cortical rotation is essential for anterior marker (Xlhbox-8) but not posterior marker (IFABP) expression.
    • Inhibition of TGF-beta signaling leads to ectopic expression of mesodermal and ectodermal markers.

    Conclusions:

    • Endodermal patterning is influenced by signaling events crucial for mesodermal development.
    • TGF-beta signaling plays a significant role in segregating the three embryonic germ layers in Xenopus.