Related Experiment Videos
A commercially available cell culture device modified for dentin barrier tests
G Schmalz1, P Garhammer, H Schweiki
1Clinic of Operative Dentistry and Periodontology, University of Regensburg, Germany.
Journal of Endodontics
|May 1, 1996
Summary
This study evaluated a dentin barrier test for dental cement cytotoxicity. Results show varying cell survival rates, with zinc phosphate cement being less toxic than glass ionomer and zinc oxide-eugenol cements.
Area of Science:
- Dental Materials Science
- Biocompatibility Testing
- Cell Biology
Background:
- Cytotoxicity assessment is crucial for dental materials.
- Existing methods may not fully replicate the in vivo environment.
- A reliable dentin barrier test is needed to evaluate material safety.
Purpose of the Study:
- To assess the suitability of a modified commercial cell culture chamber as a dentin barrier test.
- To evaluate the cytotoxicity of various dental cements using this model.
- To compare results with previous findings.
Main Methods:
- A commercial cell culture chamber was adapted using a bovine dentin disk (500 µm thick) as a barrier.
- Mouse fibroblasts were cultured on the pulpal side of the dentin.
- Dental cements were placed on the cavity side, and cell viability was assessed after 24 hours.
Main Results:
- Zinc phosphate cement (2:1 ratio) showed high cell survival (~100%).
- Ketac Fil and Ketac Silver exhibited lower cell survival (24-28%).
- Light-curing glass ionomer (Vitrebond) and zinc oxide-eugenol cements resulted in complete cell death.
Conclusions:
- The adapted dentin barrier test effectively differentiates the cytotoxicity of dental cements.
- The test provides results consistent with prior investigations.
- This model offers a valuable tool for preclinical dental material safety evaluation.