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Luminal cholecystokinin and gastrin cause gallbladder contraction
E A Brotschi1, W A Vaules, E A Kahl
1Department of Surgery, Boston Department of Veterans Affairs Medical Center, Jamaica Plain, Massachusetts 02130, USA.
The Journal of Surgical Research
|May 1, 1996
Summary
Cholecystokinin (CCK) and gastrin peptides in the gallbladder lumen stimulate gallbladder contraction. This response, potentially mediated by CCK-B receptors on intrinsic nerves, is also present in bile from fed animals.
Area of Science:
- Gastroenterology
- Neurogastroenterology
- Physiology
Background:
- Cholecystokinin-8 (CCK-8) induces gallbladder contraction via a neural pathway.
- The role of other CCK-like peptides and bile in the gallbladder lumen remains to be fully elucidated.
Purpose of the Study:
- To investigate the effects of various cholecystokinin-like peptides and hepatic bile on gallbladder contraction when placed in the gallbladder lumen.
- To determine if cholecystokinin-receptor antagonists inhibit these responses.
Main Methods:
- Isolated guinea pig gallbladders were used in an organ bath setup.
- Luminal pressure was continuously monitored to measure gallbladder contraction induced by infused peptides or bile.
Main Results:
- Luminal cholecystokinin-8 (CCK-8), cholecystokinin-5 (CCK-5), and gastrin-17 (GAS-17) induced dose-dependent gallbladder contractions with similar potency.
- These contractions were partially inhibited by cholecystokinin-receptor antagonists CR1409 and loxiglumide.
- Hepatic bile from fed animals, but not fasted animals, stimulated gallbladder contraction.
Conclusions:
- Cholecystokinin and gastrin peptides within the gallbladder lumen can induce contraction, possibly via cholecystokinin-B receptors on intrinsic nerves.
- The presence of contractile substances in bile from fed animals suggests a role in postprandial gallbladder function.
- Intrinsic nerves appear to play a significant role in the gallbladder's response to luminal peptides and bile.