Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BCL2 regulates neural differentiation

K Z Zhang1, J A Westberg, E Hölttä

  • 1Department of Pathology, University of Helsinki, Haartman Institute, Finland.

Proceedings of the National Academy of Sciences of the United States of America
|April 30, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scandcleft randomized trials of primary surgery for unilateral cleft lip and palate: Speech proficiency at 10 years of age.

International journal of language & communication disorders·2022
Same author

Retraction Note: Disruption of the Bcr-Abl/Hsp90 protein complex: a possible mechanism to inhibit Bcr-Abl-positive human leukemic blasts by novobiocin.

Leukemia·2020
Same author

Short communication: Effects of different selenium supplements on rumen fermentation and apparent nutrient and selenium digestibility of mid-lactation dairy cows.

Journal of dairy science·2019
Same author

Hydroxy-selenomethionine: A novel organic selenium source that improves antioxidant status and selenium concentrations in milk and plasma of mid-lactation dairy cows.

Journal of dairy science·2017
Same author

Stanniocalcin 1 is important for poststroke functionality, but dispensable for ischemic tolerance.

Neuroscience·2012
Same author

Electrical shielding box measurement of the negative hydrogen beam from Penning ion gauge ion source.

The Review of scientific instruments·2012
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

The BCL2 protein regulates neural differentiation. Overexpressing BCL2 promotes neurite outgrowth, while reducing it inhibits differentiation in Paju cells, impacting cell morphology and specific protein expression.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cancer Research

Background:

  • The BCL2 protein is known to inhibit apoptosis.
  • Elevated BCL2 expression is observed in various differentiating tissues, including neural tissues.
  • The role of BCL2 in cell differentiation, particularly neural differentiation, remains unclear.

Purpose of the Study:

  • To investigate whether BCL2 expression is a cause or consequence of neural differentiation.
  • To elucidate the regulatory role of BCL2 in the differentiation of neural-crest-derived cells.

Main Methods:

  • Utilized the Paju human neural-crest-derived tumor cell line for in vitro studies.
  • Induced neural differentiation using phorbol 12-myristate 13-acetate.
  • Manipulated BCL2 expression via sense and antisense cDNA transfection.

Related Experiment Videos

Main Results:

  • Overexpression of BCL2 cDNA promoted extensive neurite outgrowth and increased neuron-specific enolase expression.
  • Reduced endogenous BCL2 levels (via antisense construct) inhibited spontaneous neural differentiation, leading to epithelioid morphology and nestin upregulation.
  • BCL2 manipulation did not significantly affect cell survival under normal culture conditions.

Conclusions:

  • BCL2 gene product plays a crucial role in regulating neural differentiation.
  • BCL2 acts as a positive regulator, promoting neuronal differentiation markers.
  • The study establishes a functional link between BCL2 and the differentiation process in neural cells.