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On-line Raman spectroscopy of ribonucleotides preconcentrated by capillary isotachophoresis
P A Walker1, W K Kowalchyk, M D Morris
1Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
Analytical Chemistry
|December 1, 1995
Abstract:
Normal Raman spectroscopy is used as an on-line detector for capillary isotachophoresis (ITP) of adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in phosphate buffers. Preconcentration is from a 1 x 10(-2) M phosphate buffer (pH 7.5) into a leading electrolyte of 0.1 M KCl or Na2SO4, with a terminating electrolyte of 0.1 M 4-morpholinepropane-sulfonic acid. The ribonucleotides are concentrated to above 10(-2) M at the detection window, allowing measurement of Raman spectra with 1 s integration, from starting concentrations of 5 x 10(-6) M or higher.