Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved fiber-optic chemical sensor for penicillin

B G Healey1, D R Walt

  • 1Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.

Analytical Chemistry
|December 15, 1995
PubMed
Summary

This study introduces an optical penicillin biosensor using penicillinase enzyme. The novel sensor accurately quantifies penicillin even with pH fluctuations, crucial for fermentation monitoring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzyme Kinetics in Femtoliter Arrays.

Methods in enzymology·2016
Same author

pH-Dependent fluorescence and singlet energy transfer in water-soluble polymers containing eosin and phenol red chromophores.

Journal of fluorescence·2013
Same author

Whole-saliva proteolysis and its impact on salivary diagnostics.

Journal of dental research·2011
Same author

An Autonomous Sensor and Telemetry System for Low-Level pCO(2) Measurements in Seawater.

Analytical chemistry·2011
Same author

An Autonomous Sensor and Telemetry System for Low-Level pCO(2) Measurements in Seawater.

Analytical chemistry·2011
Same author

Generating Sensor Diversity through Combinatorial Polymer Synthesis.

Analytical chemistry·2011

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Penicillin quantification is vital in pharmaceutical production and fermentation processes.
  • Existing methods may struggle with simultaneous pH variations, complicating accurate measurements.
  • Development of robust biosensors for real-time monitoring is an ongoing challenge.

Purpose of the Study:

  • To develop and characterize a novel optical biosensor for penicillin detection.
  • To enable simultaneous and independent measurement of penicillin and pH.
  • To validate the sensor's performance in a relevant biological matrix, such as fermentation broth.

Main Methods:

  • Fabrication of an optical penicillin biosensor via selective photodeposition of polymer matrices on optical imaging fibers.
  • Immobilization of penicillinase enzyme and a pH indicator within micrometer-sized particles in a hydrogel matrix.
  • Development of an array format for simultaneous detection of penicillin and pH.
  • Enzyme kinetic analysis to model sensor response across varying pH levels.

Main Results:

  • The biosensor array allows independent and simultaneous measurement of penicillin and pH.
  • The sensor accurately quantifies penicillin in the range of 0.25–10.0 mM within a pH range of 6.2–7.5.
  • The sensor successfully quantified penicillin concentration during Penicillium chrysogenum fermentation.

Conclusions:

  • The developed optical biosensor offers a reliable method for penicillin quantification, even under fluctuating pH conditions.
  • This technology has potential applications in monitoring antibiotic production and other biochemical processes.
  • The sensor's ability to perform simultaneous pH and analyte measurements enhances its utility in complex biological samples.

Related Experiment Videos