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Published on: February 20, 2015
Antigenic and restriction enzyme analysis of Campylobacter spp associated with abortion in sheep
W J Delong1, M D Jaworski, A C Ward
1Caine Veterinary Teaching and Research Center, Department of Animal and Veterinary Science, University of Idaho, Caldwell 83605, USA.
Objective:
To determine the scope of strain variations among Campylobacter spp associated with abortion in sheep.
Design:
To examine Campylobacter spp isolated from cases of abortion for biochemical, antigenic, and genetic differences.
Sample Population:
15 isolates of Campylobacter spp isolated from cases of abortion during a single lambing season.
Procedure:
Isolates were examined, using biochemical tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins (OMP), and DNA restriction enzyme analysis (REA).
Results:
Eight strain variants were detected among the 15 isolates. 14 of the isolates were C jejuni, 13 of which were biotype I and 1 biotype II, and the remaining isolate was identified as C fetus subsp fetus. Five sodium dodecyl sulfate-polyacrylamide gel electrophoresis OMP patterns had distinctive profiles for C fetus subsp fetus and C jejuni biogroup-II isolates and 3 variants within the C jejuni biogroup-I isolates. Examination of REA patterns of DNA from the 15 isolates digested with Cfo I indicated clear differences correlating with species and biogroups and 4 REA variants among biotype-I isolates.
Conclusions:
Marked antigenic and genetic heterogeneity of Campylobacter isolates were associated with ovine abortion within a defined geographic area.
Clinical Relevance:
Representatives of differing OMP and REA profile groups should be considered for incorporation in vaccines to optimize protection in this region and possibly other geographic areas.
