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F-actin forms transient perinuclear shells at the mitosis-interphase transition

B H Clubb¹, M Locke

¹Department of Zoology, University of Western Ontario, London, Canada.

Cell Motility and the Cytoskeleton

|January 1, 1996

Summary

Actin filaments (F-actin) transiently form delicate shells around nuclei during cell division and early interphase. These perinuclear F-actin structures may be crucial for nuclear shape regulation in eukaryotes.

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Area of Science:

Cell Biology
Cytoskeleton Dynamics
Nuclear Architecture

Background:

Intermediate filaments and microtubules are established regulators of nuclear shape.
The role of F-actin in nuclear shape determination is less understood, though it's briefly associated with reforming nuclei post-cell division.

Purpose of the Study:

To investigate the potential involvement of F-actin in nuclear shape regulation.
To localize F-actin in relation to the nucleus during specific cell cycle stages.

Main Methods:

Staining of vertebrate (3T3, NRK-49F) and insect epidermal cells.
Localization of F-actin using rhodamine-phalloidin.
Nuclear staining with Hoechst #33342.

Main Results:

Related Experiment Videos

F-actin was observed forming transient shells exclusively around nuclei.
These perinuclear F-actin shells were detected during nuclear reorganization in late mitosis and early interphase.
The structures were characterized by their delicacy and transience.

Conclusions:

Perinuclear F-actin shells are transiently present during specific phases of the cell cycle.
These F-actin structures may play a role in nuclear shape maintenance.
Such shells might be a general feature in eukaryotes, often overlooked due to their ephemeral nature.