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Analysis in gingival crevicular fluid of two oligopeptides derived from human hemoglobin beta-chain
K K Mäkinen1, L Sewon, P l Mäkinen
1Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, USA.
Journal of Periodontal Research
|January 1, 1996
Abstract:
HPLC on a reversed phase column, amino acid sequencing and mass spectrometry were used to determine the structure of two human gingival crevicular exudate oligopeptides (Leu-Thr-Pro-Glu-Glu-Lys-Ser-Ala-Val-Thr-Ala-Leu and Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe) which were shown to have been derived from the beta-chain of hemoglobin. These sequences may simply represent two degradation products of the beta-chain. However, their preservation in an exudate characterized by active peptidolysis may also prompt the question about their possible more specific role.