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Effect of polyene macrolide antibiotics on invertebrate tissue culture cells

N I Goldstein, P B Fisher, C P Schaffner

    The Journal of Antibiotics
    |April 1, 1977
    PubMed

    Insights

    Amphotericin B methyl ester (AME) showed lower toxicity and DNA synthesis inhibition than amphotericin B (AB) across three insect cell lines. However, Carpocapsa pomonella cells exhibited higher sensitivity to both antifungals.

    Area of Science:

    • * Insect Cell Line Research
    • * Antifungal Drug Development
    • * Molecular Biology

    Background:

    • * Polyene macrolide antibiotics, such as amphotericin B (AB), are crucial antifungal agents.
    • * Understanding their differential effects on various insect cell lines is important for potential applications.
    • * Amphotericin B methyl ester (AME) is a derivative of AB with potentially altered properties.

    Purpose of the Study:

    • * To evaluate the impact of amphotericin B (AB) and amphotericin B methyl ester (AME) on insect cell viability.
    • * To assess the effects of AB and AME on DNA synthesis in selected insect cell lines.
    • * To compare the differential responses of various insect cell lines to these two antifungal agents.

    Main Methods:

    • * Exposure of three insect cell lines—Trichoplusia ni (Tn), Carpocapsa pomonella 169 (Cp), and Aedes aegypti (Aa)—to AB and AME.
    • * Assessment of cell viability following antibiotic treatment.
    • * Measurement of DNA synthesis rates in treated cell lines.

    Main Results:

    • * Amphotericin B methyl ester (AME) demonstrated reduced toxicity and less inhibition of DNA synthesis compared to amphotericin B (AB) across all tested cell lines.
    • * Significant differences in sensitivity to both AB and AME were observed among the three insect cell lines.
    • * Carpocapsa pomonella (Cp) cells were found to be more sensitive to both AB and AME than Trichoplusia ni (Tn) and Aedes aegypti (Aa) cells.

    Conclusions:

    • * Amphotericin B methyl ester (AME) presents a potentially safer alternative to amphotericin B (AB) for applications involving insect cell lines, due to its lower toxicity.
    • * Insect cell lines exhibit distinct sensitivities to polyene macrolide antibiotics, with Cp cells being notably more susceptible.
    • * These findings are crucial for optimizing the use of AB and AME in insect cell-based research and biotechnological applications.

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