Related Experiment Videos
Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT
T Pugatsch1, A Oppenheim, S Slavin
1Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Bone Marrow Transplantation
|February 1, 1996
Abstract:
A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10(6) female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.