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Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
Filtration of human seminal specimens using LeukoSorb leukocyte-retention medium
1Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Japan.
Journal of Obstetrics and Gynaecology (Tokyo, Japan)
|December 1, 1995
Summary
LeukoSorb filtration offers superior sperm preparation compared to Percoll centrifugation, particularly for asthenospermic and pyospermic specimens. This method enhances sperm motility, viability, and reduces abnormalities for improved fertility outcomes.
Area of Science:
- Reproductive biology and andrology.
- Sperm preparation techniques.
Background:
- Sperm preparation is crucial for assisted reproductive technologies.
- Traditional methods like Percoll centrifugation have limitations.
Purpose of the Study:
- To compare the efficacy of LeukoSorb filtration and Percoll centrifugation for sperm preparation.
- To evaluate sperm quality parameters after each preparation method.
Main Methods:
- Analysis of 26 human seminal specimens using both LeukoSorb filtration and Percoll centrifugation.
- Assessment of sperm motility, viability, and morphological abnormalities post-preparation.
Main Results:
- LeukoSorb demonstrated significantly higher sperm motility, viability, and better hypo-osmotic swelling test results in normospermic and asthenospermic samples.
- LeukoSorb also showed a greater reduction in round cell concentration in pyospermic samples.
- Filtration time correlated with total sperm concentration.
Conclusions:
- LeukoSorb filtration is an effective method for preparing sperm, especially for asthenospermic and pyospermic specimens.
- LeukoSorb offers advantages over Percoll centrifugation in improving key sperm quality parameters.
