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Filtration of human seminal specimens using LeukoSorb leukocyte-retention medium
1Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Japan.
Objective:
To compare LeukoSorb filtration with Percoll centrifugation in preparing sperm.
Methods:
Each of 26 human seminal specimens were analyzed before and after two preparation methods.
Results:
In normospermic specimens (n = 11), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motility (72.7% vs 59.2%), total swollen sperm in a hypo-osmotic swelling test (84.8% vs 59.0%), (g)-type swollen sperm (37.8% vs 23.4%) and tail abnormality (20.4% vs 10.1%). In asthenospermic specimens (n = 15), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motile sperm recovery (22.2% vs 10.1%), motility (46.9% vs 23.2%), viability (75.6% vs 54.7%), total swollen sperm (84.8% vs 59.0%) and tail abnormality (42.2% vs 24.3%), although the mean percentage of head abnormality was lower with LeukoSorb than with Percoll (30.6% vs 40.2%) vs 40.2%). In pyospermic specimens (n = 10), the mean concentration of round cells after LeukoSorb decreased significantly more than with Percoll (p = 0.029). The filtration time correlated with the total sperm concentration.
Conclusion:
LeukoSorb filtration is useful for preparing asthenospermic and pyospermic seminal specimens.
