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No FISH evidence for trisomy 7 in normal or leukemic bone marrow
M Köhler1, B Johansson, S Garwicz
1Department of Clinical Genetics, University Hospital, Lund, Sweden.
Cancer Genetics and Cytogenetics
|June 1, 1996
Summary
Trisomy 7, an extra chromosome 7, was not detected in bone marrow precursor cells of children with acute lymphoblastic leukemia (ALL). This suggests chromosome 7 gain occurs later in T cell development within the thymus.
Area of Science:
- Genetics
- Oncology
- Immunology
Background:
- Trisomy 7 is a chromosomal anomaly observed in various tumors and nonneoplastic lesions.
- Previous reports indicate trisomy 7 (+7) in tumor-infiltrating and peripheral T cells, as well as in the thymus.
Purpose of the Study:
- To investigate the presence of trisomy 7 in bone marrow precursor cells.
- To determine if trisomy 7 originates in bone marrow or during T cell maturation in the thymus.
Main Methods:
- Analysis of bone marrow samples from healthy individuals and children with acute lymphoblastic leukemia (ALL).
- Interphase fluorescence in situ hybridization (FISH) using a chromosome 7 centromere-specific probe.
Main Results:
- No evidence of trisomy 7 was found in bone marrow samples from controls or ALL patients.
- Karyotypic analysis of ALL samples revealed clonal abnormalities but none with trisomy 7.
Conclusions:
- The gain of chromosome 7 in T lymphocytes does not appear to originate in bone marrow precursor cells.
- Chromosome 7 gain likely occurs after precursor cells undergo conditioning within the thymus.