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NF-kappaB p50 subunit cross-linking to DNA duplexes, containing a monosubstituted pyrophosphate internucleotide bond
E A Kubareva1, O A Fedorova, M B Gottikh
1A.N. Belozersky Institute of Physical and Chemical Biology and Department of Chemistry, Moscow State University, Russia.
Abstract:
The new express technique based on the use of BrCN to synthesize DNA duplexes, containing non-substituted or monosubstituted pyrophosphate internucleotide bonds has been proposed. Using this technique, DNA duplexes having modified internucleotide bonds between dT and dC residues in the human NF-kappaB transcription factor recognition sequence in HIV-1 (5'-GGAAAGTCCC-3') have been prepared. We demonstrate that these internucleotide bonds within the recognition site do not prevent the formation of NF-kappaB p50 subunit complex with the corresponding duplexes. The cross-linking of NF-kappaB p50 subunit to the DNA duplex containing a monosubstituted pyrophosphate internucleotide bond has been successfully performed.