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Non-radioisotopic and semi-quantitative procedure for terminal repeat amplification protocol
J H Ohyashiki1, K Ohyashiki, T Sano
1First Department of Internal Medicine, Tokyo Medical College, Japan.
Japanese Journal of Cancer Research : Gann
|April 1, 1996
Abstract:
We have used fluorescence-labeled primers and an auto-sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi-quantitative results are possible without using a photo-capture system, and 3) no radioisotope is needed.