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Structural and thermodynamic characteristics of the binding of the lambda N protein to a RNA hairpin
M Van Gilst1, W A Rees, P H von Hippel
1Department of Chemistry, University of Oregon, Eugene 97403, USA.
Nucleic Acids Symposium Series
|January 1, 1995
Abstract:
The specific binding of the lambda N protein to a 15 nucleotide RNA oligomer that forms a hairpin structure has been investigated by biophysical methods. Using fluorescence spectroscopy and equilibrium ultra-centrifugation, it was found that the N protein binds specifically to this RNA hairpin as a monomer. Circular dichroism experiments show that both the N protein and the RNA hairpin undergo structural change upon association of the complex.