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Conformational properties of oligonucleotides
1NeXstar Pharmaceuticals, Inc., Boulder, Colorado MCDB, USA.
Nucleic Acids Symposium Series
|January 1, 1995
Summary
Vast oligonucleotide libraries are created and screened using the SELEX protocol to find molecules with specific binding or enzymatic properties. These selected oligonucleotides have diverse applications in medicine, research, and understanding life's origins.
Area of Science:
- Molecular Biology
- Biochemistry
- Evolutionary Biology
Background:
- Oligonucleotide sequences can be synthesized in vast libraries.
- These libraries are screened for molecules with specific properties like high-affinity binding or enzymatic activity.
- The SELEX (Systematic Evolution of Ligands by Exponential Enrichment) protocol is a key method for identifying such molecules.
Purpose of the Study:
- To highlight the capabilities of the SELEX protocol in generating functional oligonucleotides.
- To discuss the diverse applications of SELEX-derived oligonucleotides.
- To underscore the robustness and potency of oligonucleotide conformations.
Main Methods:
- Generation of large oligonucleotide libraries.
- Screening and selection processes (SELEX protocol).
- Characterization of selected oligonucleotide properties.
Main Results:
- Identification of individual oligonucleotide molecules with high affinity/high specificity binding.
- Discovery of oligonucleotides exhibiting novel enzymatic activities.
- Demonstration of the robustness and potency of oligonucleotide conformations.
Conclusions:
- The SELEX protocol is a powerful tool for discovering functional oligonucleotides.
- SELEX-derived oligonucleotides have significant applications in diagnostics, therapeutics, and research.
- Oligonucleotides offer valuable insights into evolutionary biology and possess potent conformational properties.