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Updated: Aug 9, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
The branch site adenosine is recognized differently for the two steps of pre-mRNA splicing
C C Query1, S A Strobel, P A Sharp
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139-4307, USA.
Abstract:
The functional groups responsible for branch site identity in the two steps of pre-mRNA splicing as well as for spliceosome assembly were tested by incorporation of site-specific modifications at the branch site of a pre-mRNA. These results show that recognition of the adenosine occurs early in complex formation and that the branch site adenosine is recognized differently before the first step and for the second step. Further, direct UV cross-linking with these modified RNAs was used to identify a factor whose interaction was dependent upon the identity of the branch site nucleotide.
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