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Analysis of conserved positions in nuclear RNase P RNA
E Pagán-Ramos1, A J Tranguch, J M Nolan
1Department of Biological Chemistry, University of Michigan, Ann Arbor 40109-0606, USA.
Nucleic Acids Symposium Series
|January 1, 1995
Abstract:
Secondary structure models of eubacterial and eukaryotic nuclear RNase P RNA subunits show extensive structural similarities, allowing the identification of highly conserved nucleotide positions and molecular modeling of the enzyme-substrate complex in three dimensions. Based on this information, we present a preliminary tertiary structure model of the yeast nuclear RNase P RNA. In addition, the most conserved positions in the structure have been subjected to sequence randomization, with viable sequence variations identified by selection in vivo and characterized for phenotypic consequences.