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[Simultaneous determination of free cholesterol and total cholesteron on an AA I Technicon autoanalyzer (authors
Abstract:
A procedure for the simultaneous enzymatic determination of free cholesterol and total cholesterol on a 2 channel AA I Technicon autoanalyzer system is described. The quantitation of free cholesterol is compared with the determination by gas chromatography. The investigation reveals a high correlation between both methods. In contrast to classical procedures the proposed quantitation of free cholesterol can be performed easily and quickly for routine analyses.