Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Versatile approach to encoding combinatorial organic syntheses using chemically robust secondary amine tags

Z J Ni1, D Maclean, C P Holmes

  • 1Affymax Research Institute, Palo Alto, California 94304, USA.

Journal of Medicinal Chemistry
|April 12, 1996
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying predictive markers for survival in malignant biliary obstruction following percutaneous transhepatic biliary drainage.

Clinical radiology·2025
Same author

Machine Learning to Predict Prostate Artery Embolization Outcomes.

Cardiovascular and interventional radiology·2024
Same author

Hemolytic disease of the fetus and newborn mediated by anti-Di<sup>a</sup> in a U.S. hospital.

Immunohematology·2023
Same author

A retrospective cohort study comparing a novel, spherical, resorbable particle against five established embolic agents for uterine fibroid embolisation.

Clinical radiology·2021
Same author

Bacterial community structure and response to nitrogen amendments in Lake Shenandoah (VA, USA).

Water science and technology : a journal of the International Association on Water Pollution Research·2019
Same author

Factors Predicting a Good Symptomatic Outcome After Prostate Artery Embolisation (PAE).

Cardiovascular and interventional radiology·2018

This study introduces a novel encoding method using secondary amines for combinatorial organic synthesis. This approach enables efficient discovery of biologically active compounds from large chemical libraries.

Area of Science:

  • Organic Chemistry
  • Medicinal Chemistry
  • Chemical Biology

Background:

  • Encoded combinatorial synthesis accelerates the discovery of biologically active compounds.
  • Existing encoding methods may lack robustness or compatibility with diverse synthetic transformations.

Purpose of the Study:

  • To develop a new, robust encoding methodology for combinatorial organic synthesis.
  • To demonstrate the versatility of this method in synthesizing diverse heterocyclic compounds.

Main Methods:

  • Utilized chemically robust secondary amines as tags for encoding.
  • Incorporated amine tags into an N-[(dialkylcarbamoyl)methyl]glycine-coding oligomer.
  • Employed acidic hydrolysis for tag regeneration and dansylation for detection via reversed-phase HPLC.

Related Experiment Videos

Main Results:

  • Successfully demonstrated encoded synthesis of beta-lactams, 4-thiazolidinones, and pyrrolidines.
  • Achieved sensitive detection of tagged molecules at sub-picomole levels.
  • The encoding chemistry proved compatible with various polymer-supported reactions.

Conclusions:

  • The developed secondary amine-based encoding strategy is versatile and efficient for combinatorial synthesis.
  • This method facilitates the discovery of novel biologically active compounds.
  • The approach is suitable for generating diverse heterocyclic compound libraries.