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Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler
1Institute of Biochemistry and Molecular Physiology, University of Potsdam, Berlin, Germany.
Biosensors & Bioelectronics
|January 1, 1996
Abstract:
Hybridisation of nucleic acid oligomers to an immobilised target has been observed in real time using evanescent field technology. A biotinylated 24-mer with random sequence including the EcoRI recognition site was immobilised via streptavidin onto a grating coupler wave guide surface. Hybridisation of 22-mer, 15-mer and 8-mer was observed. Activity of restriction endonuclease EcoRI was visualised by measurement of the loss of bound DNA after incubation.