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Molecular cloning of the forkhead transcription factor HNF-3 alpha from a human pulmonary adenocarcinoma cell line
1DH Department of Toxicology, St Bartholomew's School of Medicine, London, UK.
Abstract:
We have cloned the human homologue of HNF-3 alpha from the pulmonary adenocarcinoma cell line NCI-H441. Full-length HNF-3 alpha which was found to be 2872 bp encoded a protein of 473 amino acids. The human protein is highly related to the rat and mouse homologues and the forkhead DNA binding domain is highly conserved between all species yet identified. Northern blotting with a variety of human-derived cell lines identified a single 3 kb mRNA species. RNA levels are > 10-fold higher in the H441 cells than in any of the others.
