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Nuclear division in temperature-sensitive multicellular mutants of Wangiella dermatitidis
Journal of Bacteriology
|March 1, 1979
Abstract:
Temperature-sensitive morphological mutants of Wangiella dermatitidis were stained with mithramycin, a fluorescent nuclear stain. Multicellular forms of the mutants each contained two or more nuclei, documenting that nuclear division accompanied the yeast-to-multicellular form conversion.
Insights
Temperature-sensitive mutants of Wangiella dermatitidis showed nuclear division during multicellular formation. This study documents nuclear division accompanying yeast-to-multicellular conversion in this fungus.
Area of Science:
- Mycology
- Cell Biology
- Genetics
Background:
- Wangiella dermatitidis is a dimorphic fungus.
- Understanding morphological transitions is key to fungal biology.
Purpose of the Study:
- To investigate the nuclear behavior during the yeast-to-multicellular transition in Wangiella dermatitidis.
Main Methods:
- Utilized temperature-sensitive morphological mutants of Wangiella dermatitidis.
- Employed mithramycin, a fluorescent nuclear stain, for visualization.
Main Results:
- Multicellular forms of the mutants were observed to contain two or more nuclei.
- Nuclear division was documented to occur concurrently with the yeast-to-multicellular form conversion.
Conclusions:
- Nuclear division is an integral part of the morphological transition in Wangiella dermatitidis.
- This finding provides insight into the cell cycle regulation during fungal development.