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Immunohistochemical study of glutathione S-transferase-pi in meningiomas
M Nakamura1, S Tsunoda, T Sakaki
1Department of Neurosurgery, Nara Medical University, Japan.
Abstract:
Immunostaining for glutathione S-transferase-pi was investigated in various subtypes of meningioma for the purpose of biological characterization. Specimens included five normal meninges and 25 meningiomas (10 meningothelial type, 6 fibrous type, 5 transitional type, 3 microcystic type, and 1 secretory type). In the meningothelial type, most cells showed strongly positive staining. In the fibrous type, all cells were negative. In the transitional type, only the meningothelial components were positive. In the microcystic type, meningothelial cell clusters and arachnoid trabecular cells were positive. In the secretory type, the meningothelial components and the pseudopsammoma-body-producing cells were positive. These results suggest that the meningothelial type and the fibrous type have a different basis of development and biological features. The results also suggest that arachnoid trabecular cells and meningothelial cells share the same origin, and that the arachnoid trabecular cells serve as supportive cells and as cells which detoxify harmful substances in the subarachnoid space. The pseudopsammoma-body-producing cells in the secretory type represent the outcome of epithelial differentiation of meningothelial cells with their biological character being preserved.
Insights
Glial S-transferase-pi (GSTP) immunostaining reveals distinct biological features in meningioma subtypes. Fibrous meningiomas are GSTP-negative, unlike meningothelial subtypes, suggesting different developmental origins.
Area of Science:
- Neuroscience
- Oncology
- Cell Biology
Background:
- Meningiomas are tumors arising from the meninges.
- Understanding meningioma subtypes is crucial for diagnosis and treatment.
- Glial S-transferase-pi (GSTP) is an enzyme involved in cellular detoxification.
Purpose of the Study:
- To biologically characterize various meningioma subtypes using GSTP immunostaining.
- To investigate the potential shared origin of meningothelial and arachnoid trabecular cells.
- To explore the cellular origin of pseudopsammoma bodies in secretory meningiomas.
Main Methods:
- Immunohistochemical staining for GSTP was performed.
- Specimens included normal meninges and 25 meningiomas across different subtypes.
- Staining patterns were analyzed in meningothelial, fibrous, transitional, microcystic, and secretory meningiomas.
Main Results:
- Meningothelial meningiomas showed strong GSTP positivity.
- Fibrous meningiomas were entirely GSTP-negative.
- Transitional, microcystic, and secretory subtypes exhibited variable GSTP staining in specific cell populations.
Conclusions:
- GSTP expression patterns differentiate meningothelial and fibrous meningiomas, suggesting distinct developmental pathways.
- Arachnoid trabecular cells and meningothelial cells likely share a common origin.
- Pseudopsammoma body-producing cells in secretory meningiomas represent differentiated meningothelial cells.