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Bioprocessing with genetically modified and other organisms: case studies in processing constraints
M Moo-Young1, Y Chisti, Z Zhang
1Department of Chemical Engineering, University of Waterloo, Ontario, Canada.
Annals of the New York Academy of Sciences
|May 15, 1996
Summary
Recombinant bioprocessing requires careful bioreactor design due to genetically modified organisms' reduced shear tolerance. Process modifications are essential for efficient downstream recovery of rDNA products from these altered strains.
Area of Science:
- Biotechnology
- Bioprocessing Engineering
- Genetic Engineering
Background:
- Gene stability is crucial in recombinant cultures.
- Genetically modified organisms often exhibit reduced shear tolerance compared to wild strains.
- Recombinant DNA (rDNA) product expression influences downstream processing.
Purpose of the Study:
- To highlight processing constraints in bioprocessing genetically modified organisms.
- To discuss considerations for bioreactor design and scale-up with recombinant cultures.
- To emphasize the need for modifications in bioprocessing schemes for genetically modified strains.
Main Methods:
- Analysis of bioprocessing schemes for recombinant cultures.
- Evaluation of bioreactor design parameters considering shear tolerance.
- Assessment of downstream recovery methods impacted by rDNA expression.
Main Results:
- Genetically altered biocatalysts show reduced shear tolerance, impacting bioreactor design.
- rDNA product expression characteristics necessitate tailored downstream recovery.
- Standard bioprocessing for wild strains often requires significant adaptation for genetically modified varieties.
Conclusions:
- Bioprocessing of recombinant cultures demands specific attention to shear sensitivity.
- Downstream processing must account for the unique expression profiles of rDNA products.
- Adapting existing bioprocessing machinery is critical for successful utilization of genetically modified organisms.