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Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution
P G Penketh1, K Shyam, C L Patton
1Department of Pharmacology and Developmental Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
Analytical Biochemistry
|June 15, 1996
Summary
This study presents a simple colorimetric assay strategy to monitor pH changes in aqueous solutions. The method uses pH indicators and buffers with matching pKa values for accurate measurements.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Monitoring hydrogen ion concentration (pH) is crucial in various chemical and biological processes.
- Existing methods for pH monitoring can be complex or require specialized equipment.
Purpose of the Study:
- To develop a simple, versatile colorimetric assay for tracking pH changes.
- To demonstrate the applicability of the assay across a pH range of 3-10.
Main Methods:
- Designing assays using pH indicators and buffering agents with closely matched pKa values.
- Measuring changes in light absorption to quantify pH shifts.
- Applying the method to diverse chemical processes.
Main Results:
- Successfully developed a straightforward colorimetric assay strategy.
- Demonstrated the assay's effectiveness in monitoring processes involving net hydrogen ion generation or consumption.
- Validated the technique across the specified pH range.
Conclusions:
- The described colorimetric assay offers a simple and versatile approach for pH monitoring.
- This method is applicable to a wide range of aqueous processes within the 3-10 pH range.
- The strategy provides a cost-effective alternative for real-time process analysis.