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Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution
P G Penketh1, K Shyam, C L Patton
1Department of Pharmacology and Developmental Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
Abstract:
A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen ions in aqueous solutions over the pH range of 3-10 is described. This procedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjected to a small change in pH. Buffers and indicators are chosen with closely matching pKa values. The versatility of this type of assay technique is illustrated using three examples.
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