Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping genomic library clones using oligonucleotide arrays

R J Sapolsky1, R J Lipshutz

  • 1Affymetrix, 3380 Central Expressway, Santa Clara, California, 95051, USA.

Genomics
|May 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applications of high-density oligonucleotide arrays.

Novartis Foundation symposium·2000
Same author

Parallel genotyping of human SNPs using generic high-density oligonucleotide tag arrays.

Genome research·2000
Same author

Genome-wide mapping with biallelic markers in Arabidopsis thaliana.

Nature genetics·1999
Same author

High-throughput polymorphism screening and genotyping with high-density oligonucleotide arrays.

Genetic analysis : biomolecular engineering·1999
Same author

High density synthetic oligonucleotide arrays.

Nature genetics·1999
Same author

Mutation detection by ligation to complete n-mer DNA arrays.

Genome research·1998
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

Researchers created a new DNA probe array method to order genomic clones. This approach uses sequence markers and avoids gel-based techniques for efficient library ordering and contig mapping.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Ordering clones from genomic libraries is crucial for genome assembly.
  • Existing methods often rely on gel electrophoresis or prior sequence data, which can be limiting.

Purpose of the Study:

  • To develop a novel, high-throughput method for ordering genomic clones.
  • To create a system that bypasses the need for gel-based techniques and pre-existing sequence information.

Main Methods:

  • Utilized a high-density DNA probe array with enzymatic steps to capture dispersed sequence markers.
  • Employed Type IIS restriction sites for adapter ligation and Polymerase Chain Reaction (PCR) for marker amplification and labeling.
  • Developed informatic methods to analyze marker sets and measure clone overlap for contig mapping.

Related Experiment Videos

Main Results:

  • Successfully determined characteristic sequence marker sets for individual genomic clones.
  • Quantified clone overlap based on the similarity of their marker sets.
  • Demonstrated the ability to sequentially order clones into a contig map.

Conclusions:

  • The developed DNA probe array and associated methods provide an efficient way to order genomic clones.
  • This approach is adaptable to automation and eliminates the requirement for gel-based methods or prior sequence knowledge.