Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fast local superfusion technique

N S Veselovsky1, F Engert, H D Lux

  • 1Department of General Physiology of the Nervous System, A. A. Bogomoletz Institute of Physiology, Kiev 252601, Ukraine.

Pflugers Archiv : European Journal of Physiology
|June 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emergence of Functional Heart-Brain Circuits in a Vertebrate.

bioRxiv : the preprint server for biology·2025
Same author

Adapter CAR T cells to counteract T-cell exhaustion and enable flexible targeting in AML.

Leukemia·2023
Same author

ANALYSIS OF QUANTAL PARAMETERS OF GABA RELEASE DURING SHORT-TERM DEPRESSION AND FACILITATION OF SYNAPTIC TRANSMISSION.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same author

EXCITABILITY PROPERTIES OF TRIGEMINAL GANGLION NEURONS.

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2018
Same author

[EFFECT OF HYPOXIA ON SYNAPTIC TRANSMISSION BETWEEN RETINAL GANGLION CELLS AND SUPERIOR COLLICULUS NEURONS IN COCULTURE].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2016
Same author

[EFFECT OF PEPTIDE SEMAX ON SYNAPTIC ACTIVITY AND SHORT-TERM PLASTICITY OF GLUTAMATERGIC SYNAPSES OF CO-CULTURED DORSAL ROOT GANGLION AND DORSAL HORN NEURONS].

Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)·2015
Same journal

Expression of protein kinase A catalytic subunits in healthy and diseased mouse kidneys.

Pflugers Archiv : European journal of physiology·2026
Same journal

Localization of O₂‑sensing ADO‑RGS pathway components in mouse and human kidneys under normoxia, hypoxia and renal fibrosis.

Pflugers Archiv : European journal of physiology·2026
Same journal

Cholesterol-depleting dextrin depolarizes sarcolemma: the contribution of sodium / calcium channels and membrane integrity.

Pflugers Archiv : European journal of physiology·2026
Same journal

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

Pflugers Archiv : European journal of physiology·2026
Same journal

Renal NHE3 is required to limit hypokalemia and metabolic acidosis during dietary potassium deficiency.

Pflugers Archiv : European journal of physiology·2026
Same journal

Xenin-25 improves indomethacin-induced acute gastric injury in rats.

Pflugers Archiv : European journal of physiology·2026
See all related articles

This study introduces a novel superfusion system for rapid, local drug delivery to cultured neurons. The system enables precise control and quick solution exchange for advanced neuroscience research.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • Accurate drug delivery to cultured neurons is crucial for understanding neural function.
  • Existing methods may lack the speed and precision required for dynamic cellular studies.

Purpose of the Study:

  • To develop and describe a system for rapid, local superfusion of cultured neurons and neurites with test drugs.
  • To achieve precise control over solution delivery and exchange for high-resolution experiments.

Main Methods:

  • Utilized two micropipettes for simultaneous delivery and removal of test solutions.
  • Implemented active solution removal to ensure fast pressure control and solution exchange (<1 second).
  • Adjusted superfusion pipette pressure to direct the laminar stream onto the cell layer.

Related Experiment Videos

Main Results:

  • Achieved superfusion of areas as small as 30 microns in diameter.
  • Demonstrated rapid solution exchange, enabling quick application of multiple drugs.
  • Verified solution changes occurring within milliseconds (approx. 1 ms) via liquid junction potential measurements.

Conclusions:

  • The developed superfusion system offers high speed and spatial precision for drug application in neuronal cultures.
  • This technology facilitates dynamic pharmacological studies at the cellular level.
  • Enables rapid screening of drug effects on neurons and their processes.