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The gene encoding mouse intestinal trefoil factor: structural organization, partial sequence analysis and mapping to
R Chinery1, R Poulsom, H M Cox
1Histopathology Unit, Imperial Cancer Research Fund, London, UK. chinery@icrf.icnet.uk
Gene
|June 1, 1996
Summary
Researchers determined the gene structure of murine intestinal trefoil factor (mITF), finding it highly conserved across species. This study maps the mITF gene to chromosome 17 in mice, aiding comparative genomics.
Area of Science:
- Genomics
- Molecular Biology
- Comparative Genetics
Background:
- Trefoil peptides are secretory molecules primarily found in the gastrointestinal tract.
- Intestinal trefoil factor (ITF) is a key member of this peptide family.
Purpose of the Study:
- To determine the nucleotide sequence and genomic organization of the murine intestinal trefoil factor (mITF) gene.
- To analyze conserved regulatory elements and chromosomal location of the mITF gene.
Main Methods:
- Gene sequencing to determine nucleotide sequence and genomic structure.
- Bioinformatic analysis of conserved regulatory elements (AP-1, Sp1 binding sites, heat-shock element).
- Fluorescence in situ hybridization (FISH) for chromosomal mapping.
Main Results:
- The mITF gene comprises three exons spanning 5 kb of genomic DNA.
- The genomic sequence shows high conservation with rat and human ITF genes.
- Regulatory elements including AP-1 and Sp1 binding sites were identified.
- The mITF gene was localized to chromosome 17 in the murine genome.
Conclusions:
- The characterized mITF gene structure and conserved elements provide insights into its regulation.
- The chromosomal assignment of mITF to chromosome 17 highlights synteny with the human trefoil gene cluster on chromosome 21q22.3.
- This research contributes to understanding trefoil peptide gene evolution and function.