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Antibodies raised against guanosine bind to double-stranded DNA
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Biochemistry and Molecular Biology International
|October 1, 1995
Abstract:
Antibodies elicited against guanosine have been reported to bind to single-stranded DNA. Using an avidin-biotin microELISA, we report that these antibodies also bind to double-stranded DNA. The binding is specific and is completely inhibited by the homologous hapten. The cross-reactivity of double-stranded DNA binding antibodies to single-stranded DNA is low. The antibodies are shown to bind to the topoisomers of plasmid DNA as assessed by a gel retardation assay.