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Updated: Aug 20, 2026

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Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Published on: June 19, 2012
Northern analysis of highly folded goat alpha S1 casein mRNA
M Jansà Pérez1, C Leroux, A Sànchez Bonastre
1Departament de Patologia i Producció Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. Marta@guara.uab.es
Genetic Analysis : Biomolecular Engineering
|January 1, 1996
Abstract:
Northern blotting using glyoxal to denature a highly folded mRNA, such as goat alpha S1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.
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