[Mechanism of labilization of Penicillium vitale glucose oxidase]

The article deals with conditions for splitting the Penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and FAD as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. Specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and FAD, that is due to oxidation of the apoenzyme sulphydryl groups. Possible participation of sulphydryl groups in adding FAD to apoenzyme is under discussion.