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Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA
1Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
Molecular Biotechnology
|December 1, 1995
Abstract:
The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.