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Dynamin and receptor-mediated endocytosis
1Dept. of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
FEBS Letters
|June 24, 1996
Summary
Cell surface receptors concentrate in membrane areas, forming clathrin-coated vesicles for ligand internalization. The GTPase dynamin protein is crucial for constricting these coated pits during vesicle formation.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Receptor-mediated endocytosis is vital for cellular processes.
- Clathrin-coated vesicles mediate the internalization of cell surface receptors and bound ligands.
- This process involves complex protein machinery for vesicle formation.
Purpose of the Study:
- To review the role of proteins in clathrin-coated vesicle formation.
- To highlight the specific function of the GTPase dynamin in this process.
Main Methods:
- Literature review of endocytosis and vesicle formation.
- Focus on the molecular mechanisms of protein involvement.
Main Results:
- Receptor-ligand complexes concentrate at specialized plasma membrane regions.
- Clathrin-coated vesicles form through invagination, constriction, and budding.
- Dynamin, a GTPase, is essential for the constriction phase of coated pit formation.
Conclusions:
- The coordinated action of multiple proteins is required for efficient vesicle formation.
- Dynamin plays a critical, mechanochemical role in severing the vesicle neck during endocytosis.