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Angiotensin II and basic fibroblast growth factor induce neonatal bladder stromal cell mitogenesis
E Y Cheng1, T Grammatopoulos, C Lee
1Division of Urology, Children's Memorial Hospital, Chicago, Illinois 60614, USA.
The Journal of Urology
|August 1, 1996
Summary
Angiotensin II and basic fibroblast growth factor stimulate neonatal bladder stromal cell growth in the presence of serum factors. These growth factors require serum for their mitogenic effects on bladder stromal cells in vitro.
Area of Science:
- Urology
- Cell Biology
- Developmental Biology
Background:
- Neonatal bladder stromal cells are crucial for bladder development and function.
- Understanding the regulation of bladder stromal cell proliferation is important for studying bladder growth and repair.
Purpose of the Study:
- To establish primary stromal cell cultures from neonatal rabbit bladders.
- To investigate the mitogenic effects of angiotensin II and basic fibroblast growth factor on these cells.
Main Methods:
- Primary bladder stromal cells were isolated from 3-day-old rabbits.
- Cells were growth-arrested and stimulated with angiotensin II or basic fibroblast growth factor.
- Cell proliferation and DNA synthesis ([3H] thymidine incorporation) were measured.
Main Results:
- Both angiotensin II and basic fibroblast growth factor significantly increased cell proliferation and DNA synthesis under serum-deficient conditions.
- Basic fibroblast growth factor demonstrated a more potent mitogenic effect than angiotensin II.
- Neither agent showed significant stimulatory effects under serum-free conditions.
Conclusions:
- Angiotensin II and basic fibroblast growth factor induce a mitogenic response in neonatal bladder stromal cells in vitro.
- These mitogenic effects are dependent on the presence of serum factors.
- Further research is needed to determine the in vivo role of these factors in bladder growth.