Multilayer films containing immobilized nucleic acids. Their structure and possibilities in biosensor applications

Area of Science:

• Biomaterials Science
• Nanotechnology
• Molecular Biology

Background:

• Solid support immobilization of nucleic acids is crucial for biosensor development.
• Langmuir-Blodgett (LB) and self-assembly (SA) are advanced film deposition techniques.
• Understanding nucleic acid structure and interactions within films is key for functionality.

Purpose of the Study:

• To evaluate LB and SA film technologies for nucleic acid immobilization.
• To investigate the structural integrity of immobilized DNA using different complexing agents.
• To assess the biosensing capabilities of these nucleic acid-containing films.

Main Methods:

• Nucleic acid immobilization using LB and electrostatic attraction SA techniques.
• Complex formation with cationic surfactants (LB) and polycations (SA).
• Infrared spectral studies for structural analysis and hydration studies.

Main Results:

• DNA structural integrity varied: unfolding in some LB films, preservation in others (specific LB and SA films).
• Identified atomic groups and interactions responsible for complex formation.
• Hydration studies provided insights into water binding and nucleic acid flexibility.
• Demonstrated biosensor functionality through specific reagent binding assays.

Conclusions:

• LB and SA films offer versatile platforms for nucleic acid immobilization in biosensors.
• The choice of complexing agent significantly influences DNA structural preservation.
• These films exhibit promising potential for specific molecular recognition in biosensing applications.