Related Experiment Video
Updated: Aug 14, 2026

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Cell surface polysaccharide and protein antigens of an encapsulated Porphyromonas gingivalis 16-1 strain
Abstract:
We have extracted the cell surface polysaccharide antigen (PS) and protein antigen from an encapsulated and virulent strain of Porphyromonas gingivalis 16-1. The PS was separated from lipopolysaccharide (LPS) by Sephacryl S-300 gel chromatography. It was distinguished from LPS by immunodiffusion with homologous rabbit antiserum. The PS reacted specifically with serogroup b antiserum against P. gingivalis 16-1 and Su63 strains, but not with antisera against serogroup a (ATCC 33277) or serogroup c (W83). The outer membrane protein was extracted with the Zwittergent 3-16 and sarcosyl detergents in a Tris-EDTA buffer. SDS-Page analysis revealed that the extracted protein antigen of P. gingivalis 16-1 contained 2 immunodominant proteins with molecular weights of 41 KDa and 27 KDa.
More Related Videos
12:16Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
13:47Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
Published on: May 19, 2020
Related Concept Videos
Glycocalyx and its Functions
Components of...
Outer Layers of the Cell Envelope
Surface Appendages of Archaea
Formation of Lipopolysaccharides
Determinants of Bacterial Pathogenicity and Virulence
Streptococcal Pharyngitis