Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A systematic study of hypothermic lung preservation solutions: Euro-Collins solution

G Wu1, F Zhang, R K Salley

  • 1Department of Surgery, University of Kentucky Medical Center, Lexington, USA.

The Annals of Thoracic Surgery
|August 1, 1996
PubMed
Summary

Euro-Collins solution effectively preserves rat lungs for 4 hours at 4°C. Longer preservation times (6-24 hours) significantly impair lung function, making them unsuitable for transplantation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Cardiac syndrome X and insulin resistance].

Zhonghua nei ke za zhi·2002
Same author

[The clinical significance of changes in plasma levels of alpha-granule membrane protein and D-dimer in patients with unstable angina].

Zhonghua nei ke za zhi·2002
Same author

[Value of P-glycoprotein and glutathione S-transferase-pi as chemo-resistant indicators in ovarian cancers].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2002
Same author

[Current status of pain relief with Chinese herbal drugs in patients with cancer].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002
Same author

[Investigation of urinary interleukin-6 level in chronic renal failure patients and the influence of Rheum palmatum in treating it].

Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine·2002
Same author

Accelerating flap maturation by vascular endothelium growth factorin a rat tube flap model.

British journal of plastic surgery·2002

Area of Science:

  • Cardiovascular and Respiratory System Research
  • Organ Preservation Techniques
  • Transplantation Science

Background:

  • Lack of a suitable model hinders lung function testing post-preservation.
  • Effectiveness of various lung preservation solutions remains unconfirmed.
  • This study evaluates Euro-Collins solution for hypothermic rat lung preservation.

Purpose of the Study:

  • To assess the efficacy of Euro-Collins solution for hypothermic preservation of rat lungs.
  • To determine the optimal preservation time for maintaining lung viability.
  • To establish functional limits for Euro-Collins solution in lung preservation.

Main Methods:

  • Utilized a living rat perfusion model for isolated lung function studies.
  • Flushed rat lungs with 4°C Euro-Collins solution for preservation.

Related Experiment Videos

  • Preserved lungs for durations of 4, 6, 8, 12, and 24 hours.

    • Main Results:

    • Optimal lung function observed in control and 4-hour preservation groups.
    • Pulmonary arterial blood flow and oxygen tension were highest in the 4-hour group.
    • Lung function progressively declined with preservation times exceeding 4 hours, with severe impairment after 12 hours.

    Conclusions:

    • Euro-Collins solution effectively preserves rat lungs for up to 4 hours at 4°C.
    • Preservation for 6-8 hours led to depressed lung function.
    • Preservation beyond 12 hours rendered lungs unsuitable for transplantation due to deficient function.