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In vitro measurement of specific rat IgE antibodies
A radioimmunoassay has been developed for measuring specific rat IgE antibodies. The method used in base on the principle of the paper radio allergo sorbent technique (PRAST) as described by Wide et al. [11]. There is a good correlation between PRAST results and passive cutaneous anaphylaxis (PCA) titer. Inhibition tests with different purified monoclonal immunoglobulins show the specificity of this in vitro technique for measuring IgE antibodies. PRAST is in general somewhat more sensitive than the PCA test.
A radioimmunoassay has been developed for measuring specific rat IgE antibodies. The method used in base on the principle of the paper radio allergo sorbent technique (PRAST) as described by Wide et al. [11]. There is a good correlation between PRAST results and passive cutaneous anaphylaxis (PCA) titer. Inhibition tests with different purified monoclonal immunoglobulins show the specificity of this in vitro technique for measuring IgE antibodies. PRAST is in general somewhat more sensitive than the PCA test.