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Quantification of vanadium in serum by electrothermal atomic absorption spectrometry
1Institut für Laboratoriumsmedizin, Klinik an der Technischen Universität, München, Germany.
Clinical Chemistry
|August 1, 1996
Summary
This study presents a sensitive method for measuring vanadium in human serum using electrothermal atomic absorption spectrometry. The new technique offers reliable vanadium quantification for human health studies.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Biochemistry
Background:
- Vanadium is an essential trace element with potential health implications.
- Accurate measurement of vanadium in biological samples is crucial for research.
Purpose of the Study:
- To develop and validate an ultrasensitive and reliable method for determining vanadium in human serum.
- To establish reference ranges for vanadium in a human population.
Main Methods:
- Electrothermal atomic absorption spectrometry (ETAAS) was employed.
- Serum samples underwent lyophilization, high-pressure acid digestion, and cupferron complexation/extraction.
- A 15-fold concentration step using formic acid and injection of large aliquots enhanced sensitivity.
Main Results:
- The median vanadium concentration in 108 individuals was 50 ng/L.
- The method achieved a characteristic mass of 28 pg, limit of detection of 11 ng/L, and limit of quantification of 17 ng/L.
- High precision was demonstrated with a total imprecision (CV) of 5.5% at 1.54 µg/L and accurate recovery from a certified reference material.
Conclusions:
- The developed ETAAS method is ultrasensitive, reliable, and suitable for determining vanadium in human serum.
- The results align with previous findings, providing a valuable tool for toxicological and nutritional research.